Abstract
The granulocyte-macrophage progenitor (CFU-GM) is a multipotent cell that can differentiate to osteoclasts (OCLs), macrophages, or granulocytes. However, the relative potential of CFU-GM to efficiently form OCLs is unknown. In this report we demonstrate that granulocyte-macrophage colony-forming unit (CFU-GM)-derived cells represent an easily obtainable highly purified source of human OCL precursors that form OCLs at very high efficiency (greater than 90%) when cultured with RANK ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and dexamethasone. The OCLs that formed have high bone-resorbing activity and form multiple resorption lacunae per OCL on dentin slices. Similarly, murine marrow-derived CFU-GM also formed OCLs at a high efficiency (>80%) when treated with RANKL, M-CSF, and dexamethasone. In contrast, more committed macrophage colony-forming unit (CFU-M)-derived cells form few OCLs under these conditions.
Copyright 2000 Academic Press.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow Cells / cytology*
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Bone Resorption
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Carrier Proteins / pharmacology
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cells, Cultured
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Colony-Forming Units Assay
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Cytokines / pharmacology
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Dentin
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Dexamethasone / pharmacology
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Granulocytes / cytology
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / drug effects
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Humans
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Macrophage Colony-Stimulating Factor / pharmacology
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Macrophages / cytology
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Membrane Glycoproteins / pharmacology
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Mice
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Osteoblasts / cytology*
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Osteoblasts / drug effects
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RANK Ligand
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Receptor Activator of Nuclear Factor-kappa B
Substances
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Carrier Proteins
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Cytokines
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Membrane Glycoproteins
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RANK Ligand
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Receptor Activator of Nuclear Factor-kappa B
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TNFRSF11A protein, human
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TNFSF11 protein, human
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Tnfrsf11a protein, mouse
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Tnfsf11 protein, mouse
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Dexamethasone
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Macrophage Colony-Stimulating Factor