CFU-GM-derived cells form osteoclasts at a very high efficiency

Biochem Biophys Res Commun. 2000 Jan 27;267(3):943-6. doi: 10.1006/bbrc.1999.2042.

Abstract

The granulocyte-macrophage progenitor (CFU-GM) is a multipotent cell that can differentiate to osteoclasts (OCLs), macrophages, or granulocytes. However, the relative potential of CFU-GM to efficiently form OCLs is unknown. In this report we demonstrate that granulocyte-macrophage colony-forming unit (CFU-GM)-derived cells represent an easily obtainable highly purified source of human OCL precursors that form OCLs at very high efficiency (greater than 90%) when cultured with RANK ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and dexamethasone. The OCLs that formed have high bone-resorbing activity and form multiple resorption lacunae per OCL on dentin slices. Similarly, murine marrow-derived CFU-GM also formed OCLs at a high efficiency (>80%) when treated with RANKL, M-CSF, and dexamethasone. In contrast, more committed macrophage colony-forming unit (CFU-M)-derived cells form few OCLs under these conditions.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology*
  • Bone Resorption
  • Carrier Proteins / pharmacology
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Cytokines / pharmacology
  • Dentin
  • Dexamethasone / pharmacology
  • Granulocytes / cytology
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Humans
  • Macrophage Colony-Stimulating Factor / pharmacology
  • Macrophages / cytology
  • Membrane Glycoproteins / pharmacology
  • Mice
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • RANK Ligand
  • Receptor Activator of Nuclear Factor-kappa B

Substances

  • Carrier Proteins
  • Cytokines
  • Membrane Glycoproteins
  • RANK Ligand
  • Receptor Activator of Nuclear Factor-kappa B
  • TNFRSF11A protein, human
  • TNFSF11 protein, human
  • Tnfrsf11a protein, mouse
  • Tnfsf11 protein, mouse
  • Dexamethasone
  • Macrophage Colony-Stimulating Factor