Roles of p38 MAPK, PKC and PI3-K in the signaling pathways of NADPH oxidase activation and phagocytosis in bovine polymorphonuclear leukocytes

FEBS Lett. 2000 Feb 11;467(2-3):253-8. doi: 10.1016/s0014-5793(00)01167-4.


Stimulation of bovine polymorphonuclear leukocytes (PMN) with serum-opsonized zymosan (sOZ) induced the activation of p38 mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3-K) and sOZ-induced O(2)(-) production was significantly attenuated by their inhibitors (SB203580 for p38 MAPK, GF109203X for PKC and wortmannin for PI3-K). They caused significant attenuation of sOZ-induced phosphorylation of p47phox as well. Flow cytometric analysis, however, revealed that SB203580 and wortmannin attenuated phagocytosis, but GF109203X facilitated it. The results suggest that p38 MAPK and PI3-K participated in both signaling pathways of NADPH oxidase activation (O(2)(-) production) and phagocytosis, and PKC participated in the signaling pathway of NADPH oxidase activation alone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cattle
  • Enzyme Activation / drug effects
  • Enzyme Inhibitors / pharmacology
  • Mitogen-Activated Protein Kinases*
  • NADPH Oxidases / metabolism*
  • Neutrophils / enzymology*
  • Phagocytosis / drug effects
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Protein Kinase C / metabolism*
  • Signal Transduction
  • Zymosan
  • p38 Mitogen-Activated Protein Kinases


  • Enzyme Inhibitors
  • Zymosan
  • NADPH Oxidases
  • Phosphatidylinositol 3-Kinases
  • Protein Kinase C
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases