Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants

FEBS Lett. 2000 Feb 11;467(2-3):273-8. doi: 10.1016/s0014-5793(00)01153-4.

Abstract

We have cloned and characterized the first member of a novel family of ammonium transporters in plants: AtAMT2 from Arabidopsis thaliana. AtAMT2 is more closely related to bacterial ammonium transporters than to plant transporters of the AMT1 family. The protein was expressed and functionally characterized in yeast. AtAMT2 transported ammonium in an energy-dependent manner. In contrast to transporters of the AMT1 family, however, AtAMT2 did not transport the ammonium analogue, methylammonium. AtAMT2 was expressed more highly in shoots than roots and was subject to nitrogen regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / biosynthesis
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Quaternary Ammonium Compounds / metabolism
  • Sequence Alignment
  • Yeasts / metabolism

Substances

  • DNA, Complementary
  • Plant Proteins
  • Quaternary Ammonium Compounds

Associated data

  • GENBANK/AF182039