A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch

FEBS Lett. 2000 Feb 11;467(2-3):348-55. doi: 10.1016/s0014-5793(00)01135-2.


We have isolated two human ubiquitin-like (UbL) proteins that bind to a short peptide within the ATPase domain of the Hsp70-like Stch protein. Chap1 is a duplicated homologue of the yeast Dsk2 gene that is required for transit through the G2/M phase of the cell cycle and expression of the human full-length cDNA restored viability and suppressed the G2/M arrest phenotype of dsk2Delta rad23Delta Saccharomyces cerevisiae mutants. Chap2 is a homologue for Xenopus scythe which is an essential component of reaper-induced apoptosis in egg extracts. While the N-terminal UbL domains were not essential for Stch binding, Chap1/Dsk2 contains a Sti1-like repeat sequence that is required for binding to Stch and is also conserved in the Hsp70 binding proteins, Hip and p60/Sti1/Hop. These findings extend the association between Hsp70 members and genes encoding UbL sequences and suggest a broader role for the Hsp70-like ATPase family in regulating cell cycle and cell death events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / metabolism
  • Amino Acid Sequence
  • Autophagy-Related Proteins
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Fungal Proteins / genetics
  • HSP70 Heat-Shock Proteins / genetics*
  • HSP70 Heat-Shock Proteins / metabolism
  • Humans
  • Molecular Sequence Data
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Ubiquitins / chemistry
  • Ubiquitins / genetics*
  • Ubiquitins / metabolism


  • Adaptor Proteins, Signal Transducing
  • Autophagy-Related Proteins
  • Cell Cycle Proteins
  • DSK2 protein, S cerevisiae
  • Fungal Proteins
  • HSP70 Heat-Shock Proteins
  • HSPA13 protein, human
  • Saccharomyces cerevisiae Proteins
  • UBQLN2 protein, human
  • Ubiquitins
  • Adenosine Triphosphatases

Associated data

  • GENBANK/AF189009