Endophilin/SH3p4 is required for the transition from early to late stages in clathrin-mediated synaptic vesicle endocytosis

Neuron. 1999 Sep;24(1):143-54. doi: 10.1016/s0896-6273(00)80828-4.


Endophilin/SH3p4 is a protein highly enriched in nerve terminals that binds the GTPase dynamin and the polyphosphoinositide phosphatase synaptojanin, two proteins implicated in synaptic vesicle endocytosis. We show here that antibody-mediated disruption of endophilin function in a tonically stimulated synapse leads to a block in the invagination of clathrin-coated pits adjacent to the active zone and therefore to a block of synaptic vesicle recycling. We also show that in a cell-free system, endophilin is not associated with clathrin coats and is a functional partner of dynamin. Our findings suggest that endophilin is part of a biochemical machinery that acts in trans to the clathrin coat from early stages to vesicle fission.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Antibodies / pharmacology
  • Caenorhabditis elegans / genetics
  • Carrier Proteins / antagonists & inhibitors
  • Carrier Proteins / chemistry
  • Carrier Proteins / physiology*
  • Cell-Free System
  • Clathrin / physiology*
  • Coated Pits, Cell-Membrane / physiology*
  • Dynamins
  • Endocytosis*
  • GTP Phosphohydrolases / physiology
  • Lampreys
  • Microscopy, Electron
  • Molecular Sequence Data
  • Rats
  • Spinal Cord / chemistry
  • Synapses / ultrastructure
  • Synaptic Vesicles / metabolism*
  • Synaptic Vesicles / ultrastructure
  • src Homology Domains


  • Adaptor Proteins, Signal Transducing
  • Antibodies
  • Carrier Proteins
  • Clathrin
  • GTP Phosphohydrolases
  • Dynamins