Near-infrared spectroscopy: does it function in functional activation studies of the adult brain?

Int J Psychophysiol. 2000 Mar;35(2-3):125-42. doi: 10.1016/s0167-8760(99)00048-3.


Changes in optical properties of biological tissue can be examined by near-infrared spectroscopy (NIRS). The relative transparency of tissues including the skull to near-infrared light is the prerequisite to apply the method to brain research. We describe the methodology with respect to its applicability in non-invasive functional research of the adult cortex. A summary of studies establishing the 'typical' response in NIRS vascular parameters, i.e. changes in the concentration of oxygenated and deoxygenated haemoglobin, over an activated area is followed by the validation of changes in the cytochrome-oxidase redox state in response to a visual stimulus. Proceeding from these findings a rough mapping of this metabolic response over the motion-sensitive extrastriate visual area is demonstrated. NIRS measures concentration changes in deoxygenated haemoglobin [deoxy-Hb] which are assumed to be the basis of fMRI BOLD contrast (blood oxygenation level-dependent). The method is therefore an excellent tool to validate assumptions on the physiological basis underlying the fMRI signal, due to its high specificity as to the parameters measured. Questions concerning the concept of 'activation'/'deactivation' and that of the linearity of the vascular response are discussed. To challenge the method we finally present results from a complex single-trial motor paradigm study testing the hypothesis, that premotor potentials (contingent negative variation) can be examined by functional techniques relying on the vascular response. Some of the work described here has been published elsewhere.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Algorithms
  • Brain / enzymology
  • Brain / physiology*
  • Cerebrovascular Circulation / physiology
  • Electron Transport Complex IV / metabolism
  • Hemoglobins / metabolism
  • Humans
  • Oxyhemoglobins / metabolism
  • Psychomotor Performance / physiology
  • Reaction Time / physiology
  • Research Design
  • Signal Transduction / physiology
  • Spectroscopy, Near-Infrared*


  • Hemoglobins
  • Oxyhemoglobins
  • Electron Transport Complex IV