The carotid body monitors arterial oxygen tension. Spectrophotometric recording of the intact organ has revealed a cytochrome aa3 and a cytochrome b558 as potential oxygen sensor candidates. The latter is known as part of the NADPH oxidase system generating superoxide anions in the "respiratory burst" defense mechanism, and glomus cells have been found to exhibit immunoreactivity against this phagocyte cytochrome b558. Using a monoclonal antibody against the large cytochrome b558 subunit, gp91phox, and other antibodies serving as neural (PGP 9.5) and monocyte/macrophage markers (ED1, ED2), we here demonstrate at light and electron microscopical level that monocytes/macrophages are abundantly present in the rat carotid body and represent the major source of cytochrome b558 in this organ. Their presence has profound implications on the interpretation of spectrophotometric recordings aimed to elucidate the mechanisms of oxygen sensing since their high cytochrome b558 content will obscure possible contributions of cell types involved in the oxygen sensor process.