Transforming growth factors beta(1) (TGF-beta(1)) and TGF-beta(2) promote glioma cell migration via Up-regulation of alpha(V)beta(3) integrin expression

Biochem Biophys Res Commun. 2000 Feb 16;268(2):607-11. doi: 10.1006/bbrc.2000.2176.


The migratory behaviour of malignant gliomas relies on the interaction of integrins with extracellular matrix (ECM) components. Transforming growth factor-beta(1) (TGF-beta(1)) potently stimulates glioma cell motility whereas TGF-beta(2) is known for its immunosuppressive properties. Here, we show that both TGF-beta(1) and TGF-beta(2) promote migration of glioma cells. In parallel, TGF-beta(1) and TGF-beta(2) induce alpha(V) and beta(3) intergrin mRNA expression and enhance cell surface expression of alpha(V)beta(3) integrin. TGF-beta-mediated promotion of migration is abrogated by echistatin, a Arg-Gly-Asp (RGD) peptide antagonist of alpha(V)beta(3) integrin, and by a neutralizing anti-alpha(V)beta(3) integrin antibody. Taken together, we report a novel mechanism by which TGF-beta modulates cell ECM interactions and promotes glioma cell motility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Movement*
  • Gene Expression Regulation
  • Glioma / pathology*
  • Humans
  • Integrins / antagonists & inhibitors
  • Integrins / biosynthesis
  • Integrins / genetics*
  • Intercellular Signaling Peptides and Proteins
  • Peptides / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Transcriptional Activation
  • Transforming Growth Factor beta / physiology*
  • Tumor Cells, Cultured
  • Up-Regulation


  • Integrins
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • RNA, Messenger
  • Transforming Growth Factor beta
  • echistatin