Dot-blot nitrocellulose enzyme immunoassays for the detection of white-spot virus and yellow-head virus of penaeid shrimp

J Virol Methods. 2000 Feb;84(2):175-9. doi: 10.1016/s0166-0934(99)00140-8.

Abstract

Dot-blot nitrocellulose enzyme immunoassays (DB-NC-EIA) were developed for the detection of white-spot virus (WSV) and yellow-head virus (YHV) in infected shrimp. The assays utilized HRP-conjugated virus-specific antibodies to detect virus antigen present in gill homogenates of infected shrimp spotted onto nitrocellulose membrane. The assays are by far the simplest and most rapid detection methods available for WSV and YHV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology
  • Antigens, Viral / analysis*
  • Baculoviridae / isolation & purification*
  • Collodion
  • Decapoda / virology*
  • Gills / virology
  • Immunoblotting / methods*
  • Immunoenzyme Techniques / methods*
  • Rabbits

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Collodion