An artificial transcriptional activating region with unusual properties

Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):1988-92. doi: 10.1073/pnas.040573197.


We describe a series of transcriptional activators generated by adding amino acids (eight in one case, six in another) to fragments of the yeast Saccharomyces cerevisiae activator Gal4 that dimerize and bind DNA. One of the novel activating regions identified by this procedure is unusual, compared with previously characterized yeast activating regions, in the following ways: it works more strongly than does Gal4's natural activating region as assayed in yeast; it is devoid of acidic residues; and several lines of evidence suggest that it sees targets in the yeast transcriptional machinery at least partially distinct from those seen by Gal4's activating region.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Molecular Sequence Data
  • Mutagenesis
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcriptional Activation*


  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/AF223921
  • GENBANK/AF223922
  • GENBANK/AF223923
  • GENBANK/AF223924
  • GENBANK/AF223925
  • GENBANK/AF223926
  • GENBANK/AF223927
  • GENBANK/AF223928
  • GENBANK/AF223929
  • GENBANK/AF223930
  • GENBANK/AF223931
  • GENBANK/AF223932
  • GENBANK/AF223933
  • GENBANK/AF223934
  • GENBANK/AF223935
  • GENBANK/AF223936