Induction of tcI 7, a gene encoding a beta-subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide

FEBS Lett. 2000 Jan 28;466(2-3):213-8. doi: 10.1016/s0014-5793(00)01084-x.

Abstract

We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tcl 7 was up-regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an up-regulation of tcI 7 by salicylic acid (SA). Moreover, accumulation of tcI 7 transcripts after treatment with cryptogein or with SA only occurred in NahG 9-tobacco plants that do not express the salicylate hydroxylase and thus are able to accumulate SA and develop a SAR. Suppressed accumulation of tcI 7 transcripts in NahG 8+ tobacco plants after cryptogein or SA treatment correlated with the loss of SAR. H2O2 was also shown to up-regulate tcI 7 in tobacco plants. Using gene walking by PCR we cloned and sequenced the 5' flanking region of tcI 7 containing hypothetical regulatory sequences, especially myb and NF-kappaB boxes, that could be responsible for the regulation of tcI 7 by salicylic acid and H2O2 respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cysteine Endopeptidases / genetics*
  • DNA, Complementary
  • Fungal Proteins / pharmacology*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Gene Expression Regulation, Plant / drug effects*
  • Hydrogen Peroxide / pharmacology*
  • Molecular Sequence Data
  • Plant Proteins*
  • Plants, Toxic*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Salicylic Acid / pharmacology*
  • Tobacco / drug effects*
  • Tobacco / genetics

Substances

  • DNA, Complementary
  • Fungal Proteins
  • Plant Proteins
  • RNA, Messenger
  • Hydrogen Peroxide
  • Cysteine Endopeptidases
  • TcI 7 protein, Nicotiana tabacum
  • Salicylic Acid