P-glycoprotein is localized in caveolae in resistant cells and in brain capillaries

FEBS Lett. 2000 Jan 28;466(2-3):219-24. doi: 10.1016/s0014-5793(00)01087-5.


A significant proportion of P-glycoprotein (P-gp) and caveolin was co-localized in caveolae isolated from resistant (CH(R)C5) cells overexpressing P-gp and from drug-sensitive Chinese hamster ovary cells (AuxB1). The proportion of P-gp and caveolin associated with caveolar microdomains was higher in CH(R)C5 cells grown in the presence of P-gp substrates (cyclosporin A or colchicine) than in untreated CH(R)C5 cells. Coimmunoprecipitation of P-gp and caveolin from CH(R)C5 lysates suggests that there is a physical interaction between them. Furthermore, co-localization of P-gp and caveolin was found in caveolae from brain capillaries, indicating that this association also takes place in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
  • Amino Acid Sequence
  • Animals
  • Brain / blood supply*
  • CHO Cells
  • Capillaries / metabolism*
  • Cricetinae
  • Humans
  • Membrane Proteins / metabolism
  • Precipitin Tests
  • Tumor Cells, Cultured


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Membrane Proteins