Genetic analysis of the cell-to-cell movement of beet yellows closterovirus

Virology. 2000 Mar 1;268(1):192-200. doi: 10.1006/viro.1999.0155.

Abstract

A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll. A series of mutations targeting genes that are not involved in amplification of the viral positive-strand RNA was analyzed. The products of genes coding for a 6-kDa hydrophobic protein (p6) and a 64-kDa protein (p64), as well as for minor and major capsid proteins, were found to be essential for intercellular translocation of BYV. In a previous work, we have demonstrated that the BYV HSP70-homolog (HSP70h) also plays a critical role in viral movement (V. V. Peremyslov, Y. Hagiwara, and V. V. Dolja, 1999, Proc. Natl. Acad. Sci. USA, 96, 14771-14776). Altogether, a unique protein quintet including three dedicated movement proteins (p6, p64, and HSP70h) and two structural proteins is required to potentiate the cell-to-cell movement of a closterovirus. The corresponding BYV genes are clustered in a block that is conserved among diverse representatives of the family Closteroviridae.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Capsid / metabolism
  • Closterovirus / genetics*
  • Closterovirus / metabolism
  • Closterovirus / physiology*
  • Green Fluorescent Proteins
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Movement
  • Plant Leaves / virology
  • Plant Viral Movement Proteins
  • Plants / virology*
  • RNA, Viral / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Viral Proteins / genetics
  • Viral Proteins / metabolism
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / metabolism

Substances

  • Luminescent Proteins
  • Plant Viral Movement Proteins
  • RNA, Viral
  • Viral Proteins
  • Viral Structural Proteins
  • Green Fluorescent Proteins