Detection of integrins in cataract lens epithelial cells

J Cataract Refract Surg. 2000 Feb;26(2):287-91. doi: 10.1016/s0886-3350(99)00369-7.


Purpose: To detect the expression of integrin subunits in lens epithelial cells (LECs) of human cataracts.

Setting: Research Laboratory, International Intraocular Implant Training Centre, Tianjin Medical University, China.

Methods: The circular sections of the anterior capsules with attached LECs were obtained during cataract surgery from 100 patients. The LECs were stained with an avidin-biotin-complex immunohistochemical technique using 5 monoclonal antibodies specific for alpha subunits 2, 3, 5 and beta subunits 1, 2.

Results: All integrin subunits studied were found to varying degrees in specimens. The positive percentages were 70%, 65%, 75%, 70%, and 80%, respectively.

Conclusion: Integrin subunits were present in LECs of human cataracts. These molecules may serve in the adhesion of LECs to the lens capsule and play a role in cell-posterior capsule interaction after cataract surgery.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal
  • Biomarkers
  • Cataract / metabolism*
  • Cataract Extraction
  • Epithelial Cells / metabolism*
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Integrins / metabolism*
  • Lens Capsule, Crystalline / metabolism*
  • Lens Capsule, Crystalline / pathology
  • Male
  • Middle Aged


  • Antibodies, Monoclonal
  • Biomarkers
  • Integrins