The small leucine-rich repeat proteoglycan biglycan binds to alpha-dystroglycan and is upregulated in dystrophic muscle

J Cell Biol. 2000 Feb 21;148(4):801-10. doi: 10.1083/jcb.148.4.801.

Abstract

The dystrophin-associated protein complex (DAPC) is necessary for maintaining the integrity of the muscle cell plasma membrane and may also play a role in coordinating signaling events at the cell surface. The alpha-/beta-dystroglycan subcomplex of the DAPC forms a critical link between the cytoskeleton and the extracellular matrix. A ligand blot overlay assay was used to search for novel dystroglycan binding partners in postsynaptic membranes from Torpedo electric organ. An approximately 125-kD dystroglycan-binding polypeptide was purified and shown by peptide microsequencing to be the Torpedo ortholog of the small leucine-rich repeat chondroitin sulfate proteoglycan biglycan. Biglycan binding to alpha-dystroglycan was confirmed by coimmunoprecipitation with both native and recombinant alpha-dystroglycan. The biglycan binding site was mapped to the COOH-terminal third of alpha-dystroglycan. Glycosylation of alpha-dystroglycan is not necessary for this interaction, but binding is dependent upon the chondroitin sulfate side chains of biglycan. In muscle, biglycan is detected at both synaptic and nonsynaptic regions. Finally, biglycan expression is elevated in muscle from the dystrophic mdx mouse. These findings reveal a novel binding partner for alpha-dystroglycan and demonstrate a novel avenue for interaction of the DAPC and the extracellular matrix. These results also raise the possibility of a role for biglycan in the pathogenesis, and perhaps the treatment, of muscular dystrophy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biglycan
  • Binding Sites
  • Chondroitin Sulfates / metabolism
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / metabolism*
  • Dystroglycans
  • Dystrophin / deficiency
  • Dystrophin / genetics
  • Dystrophin / physiology
  • Extracellular Matrix Proteins
  • Glycosylation
  • Humans
  • Leucine / analysis*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Molecular Weight
  • Muscles / chemistry
  • Muscles / metabolism*
  • Muscles / pathology
  • Muscular Dystrophies / genetics
  • Muscular Dystrophies / metabolism*
  • Muscular Dystrophies / pathology
  • Neuromuscular Junction / chemistry
  • Protein Binding
  • Proteoglycans / chemistry
  • Proteoglycans / genetics
  • Proteoglycans / isolation & purification
  • Proteoglycans / metabolism*
  • Repetitive Sequences, Amino Acid
  • Synapses / chemistry
  • Torpedo
  • Up-Regulation*

Substances

  • BGN protein, human
  • Bgn protein, mouse
  • Biglycan
  • Cytoskeletal Proteins
  • DAG1 protein, human
  • Dystrophin
  • Extracellular Matrix Proteins
  • Membrane Glycoproteins
  • Proteoglycans
  • Dystroglycans
  • Chondroitin Sulfates
  • Leucine