Creamatocrit and the nutrient composition of human milk

J Perinatol. Jul-Aug 1999;19(5):343-6. doi: 10.1038/


Objective: We tested the hypothesis that creamatocrit, the length of the cream column separated from milk by centrifugation and expressed as a percentage of the length of the total milk column, is a useful measure of the lipid concentration and the energy content of human milk.

Study design: Milk samples from 17 mothers of preterm infants were analyzed prospectively, fresh as well as frozen and thawed, for creamatocrit measurement and nutrient composition.

Results: Creamatocrit correlated strongly with lipid concentration and energy content of human milk, fresh or frozen and thawed. The energy content can be calculated from the regression equation: Energy (kcal/dl) = 5.99 x creamatocrit(%) + 32.5 for a fresh sample, and energy (kcal/dl) = 6.20 x creamatocrit(%) + 35.1 for a frozen sample.

Conclusion: Calculations of energy content from the creamatocrit measurement may be useful for an accurate assessment of energy intake in preterm infants fed human milk.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Centrifugation
  • Dietary Fats*
  • Energy Intake
  • Female
  • Freezing
  • Humans
  • Infant, Newborn
  • Infant, Premature
  • Lactation*
  • Lipids / analysis
  • Milk / chemistry*
  • Mothers
  • Nutritive Value
  • Prospective Studies
  • Regression Analysis


  • Dietary Fats
  • Lipids