Relationship between disease duration and predominant orbital T cell subset in Graves' ophthalmopathy

J Clin Endocrinol Metab. 2000 Feb;85(2):776-80. doi: 10.1210/jcem.85.2.6333.


We sought to determine whether the predominant orbital T helper (T(H)) cell subset in orbital T cell clones established from patients with Graves' ophthalmopathy (GO) might be related to disease duration. A total of 117 clones were established from orbital adipose/connective tissues of 6 GO patients, and cytokine production was measured in 57 CD3+CD4+ clones. T(H)1-type clones were predominant in cultures from patients with recent onset (<2 yr) Graves' hyperthyroidism (n = 44; TH1/TH0/TH2 = 57/29/14%) or GO (n = 53 clones; TH1/TH0/TH2 = 47/30/23%). In contrast, TH2-type clones predominated in cultures from patients with more remote onset (>2 yr) hyperthyroidism (n = 13; TH1/TH0/TH2 = 0/31/69%; P < 0.005) or GO (n = 4; TH1/TH0/TH2 = 0/25/75%; P = 0.05). In addition, we established T cell clones from 1 TH1-dominant patient with recent-onset thyroid and eye disease using either IL-2 (12.5 ng/mL) alone or IL-2 plus IL-4 (5 ng/mL) and found no shift toward recovery of TH2-type clones in the latter. In conclusion, although the CD3+CD4+ clones characterized were not necessarily tissue antigen specific, our findings suggest that cell-mediated (TH1-type) immune reactions may predominate in the orbit in early GO, whereas humoral immunity (TH2-type) might play the greater role in later stages of the disease.

MeSH terms

  • Adipose Tissue / pathology
  • Adult
  • Aged
  • Cells, Cultured
  • Connective Tissue / pathology
  • Cytokines / biosynthesis
  • Female
  • Graves Disease / pathology*
  • Humans
  • Male
  • Middle Aged
  • Orbit / pathology*
  • Phenotype
  • T-Lymphocyte Subsets / pathology*
  • T-Lymphocyte Subsets / physiology
  • Th1 Cells / pathology
  • Th2 Cells / pathology
  • Time Factors


  • Cytokines