Human B-cell lines constitutively express and secrete interleukin-16

Immunology. 2000 Feb;99(2):266-71. doi: 10.1046/j.1365-2567.2000.00959.x.


Interleukin-16 (IL-16), produced by activated CD8+ T lymphocytes, is inhibitory to human immunodeficiency virus-1 (HIV-1) replication. In an attempt to determine whether human B cells express and secrete IL-16, a wide panel of B-cell lines derived from patients with acquired immune deficiency syndrome (AIDS)-associated B-cell lymphomas (AABCL) (n = 5) and from non-AABCLs (n = 8) were studied. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we were able to observe ubiquitous expression of IL-16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL-16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL-16 secretion by human B-cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / immunology*
  • Gene Expression
  • Humans
  • Interleukin-16 / genetics
  • Interleukin-16 / metabolism*
  • Lymphoma, B-Cell / immunology*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Interleukin-16
  • RNA, Messenger
  • RNA, Neoplasm