Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization

Cell. 2000 Feb 18;100(4):399-409. doi: 10.1016/s0092-8674(00)80676-5.


Asymmetric localization is a prerequisite for inscuteable (insc) to function in coordinating and mediating asymmetric cell divisions in Drosophila. We show here that Partner of Inscuteable (Pins), a new component of asymmetric divisions, is required for Inscuteable to asymmetrically localize. In the absence of pins, Inscuteable becomes cytoplasmic and asymmetric divisions of neuroblasts and mitotic domain 9 cells show defects reminiscent of insc mutants. Pins colocalizes with Insc and interacts with the region necessary and sufficient for directing its asymmetric localization. Analyses of pins function in neuroblasts reveal two distinct steps for Insc apical cortical localization: A pins-independent, bazooka-dependent initiation step during delamination (interphase) and a later maintenance step during which Baz, Pins, and Insc localization are interdependent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Cycle Proteins*
  • Cell Differentiation
  • Cytoskeletal Proteins / metabolism*
  • DNA, Complementary
  • Drosophila / embryology
  • Drosophila / genetics
  • Drosophila / metabolism
  • Drosophila Proteins*
  • Humans
  • Insect Proteins / genetics
  • Insect Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Neurons / metabolism
  • Neuropeptides
  • Rabbits
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Spindle Apparatus
  • Stem Cells / metabolism


  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • DNA, Complementary
  • Drosophila Proteins
  • Insect Proteins
  • Neuropeptides
  • Pins protein, Drosophila
  • Recombinant Fusion Proteins
  • insc protein, Drosophila

Associated data

  • GENBANK/AF230071