Ras1 promotes cellular growth in the Drosophila wing

Cell. 2000 Feb 18;100(4):435-46. doi: 10.1016/s0092-8674(00)80679-0.


The Ras GTPase links extracellular mitogens to intracellular mechanisms that control cell proliferation. To understand how Ras regulates proliferation in vivo, we activated or inactivated Ras in cell clones in the developing Drosophila wing. Cells lacking Ras were smaller, had reduced growth rates, accumulated in G1, and underwent apoptosis due to cell competition. Conversely, activation of Ras increased cell size and growth rates and promoted G1/S transitions. Ras upregulated the growth driver dMyc, and both Ras and dMyc increased levels of cyclin E posttranscriptionally. We propose that Ras primarily promotes growth and that growth is coupled to G1/S progression via cyclin E. Interestingly, upregulation of growth by Ras did not deregulate G2/M progression or a developmentally regulated cell cycle exit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Apoptosis
  • Cell Differentiation
  • Cell Division
  • Cell Survival
  • Cyclin E / metabolism
  • DNA-Binding Proteins*
  • Drosophila / growth & development*
  • Drosophila Proteins*
  • G1 Phase
  • Insect Proteins / metabolism
  • S Phase
  • Time Factors
  • Transcription Factors / metabolism
  • Up-Regulation
  • Wings, Animal / growth & development
  • ras Proteins / genetics
  • ras Proteins / physiology*


  • Cyclin E
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Insect Proteins
  • Myc protein, Drosophila
  • Transcription Factors
  • ras Proteins