Activation of the human p27(Kip1) promoter by IFNalpha 2b

Biochem Biophys Res Commun. 2000 Mar 5;269(1):31-4. doi: 10.1006/bbrc.2000.2256.

Abstract

p27(Kip1) is one of the key regulatory proteins in cell cycle through inhibition of pRB phosphorylation by suppression of the activity of several cyclin/Cdk complexes. The expression of p27(Kip1) has been shown to be controlled by a posttranslational mechanism, although vitamin D(3) and neuronal differentiation can also induce its mRNA. Recently, the p27(Kip1) promoter was isolated and sequenced from a human leukocyte genomic library. In this report, we demonstrate that IFNalpha 2b, activates the human p27(Kip1) promoter-driven luciferase reporter gene in transient expression assays in H82 cells. This induction might involve two IRF 1-like binding sites present in the p27(Kip1) promoter. To our knowledge this is the first report on the direct activation of the human p27(Kip1) promoter by IFNalpha 2b.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites / genetics
  • Cell Cycle Proteins*
  • Cell Line
  • Cyclin-Dependent Kinase Inhibitor p27
  • DNA / genetics
  • DNA / metabolism
  • DNA Primers / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Genes, Reporter / drug effects
  • Humans
  • Interferon Regulatory Factor-1
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Luciferases / genetics
  • Microtubule-Associated Proteins / genetics*
  • Molecular Sequence Data
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Promoter Regions, Genetic / drug effects*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Proteins
  • Transcriptional Activation / drug effects
  • Transfection
  • Tumor Suppressor Proteins*

Substances

  • Cell Cycle Proteins
  • DNA Primers
  • DNA-Binding Proteins
  • IRF1 protein, human
  • Interferon Regulatory Factor-1
  • Interferon alpha-2
  • Interferon-alpha
  • Microtubule-Associated Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Recombinant Proteins
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27
  • DNA
  • Luciferases