A novel anionic inward rectifier in native cardiac myocytes

Circ Res. 2000 Mar 3;86(4):E63-71.

Abstract

Although the cationic inward rectifiers (Kir and hyperpolarization-activated I(f) channels) have been well characterized in cardiac myocytes, the expression and physiological role of anionic inward rectifiers in heart are unknown. In the present study, we report the functional and molecular identification of a novel chloride (Cl(-)) inward rectifier (Cl.ir) in mammalian heart. Under conditions in which cationic inward rectifier channels were blocked, membrane hyperpolarization (-40 to -140 mV) activated an inwardly rectifying whole-cell current in mouse atrial and ventricular myocytes. Under isotonic conditions, the current activated slowly with a biexponential time course (time constants averaging 179.7+/-23.4 [mean+/-SEM] and 2073.6+/-287.6 ms at -120 mV). Hypotonic cell swelling accelerated the activation and increased the current amplitude whereas hypertonic cell shrinkage inhibited the current. The inwardly rectifying current was carried by Cl(-) (I(Cl.ir)) and had an anion permeability sequence of Cl(-)>I(-)>>aspartate. I(Cl.ir) was blocked by 9-anthracene-carboxylic acid and cadmium but not by stilbene disulfonates and tamoxifen. A similar I(Cl.ir) was also observed in guinea pig cardiac myocytes. The properties of I(Cl.ir) are consistent with currents generated by expression of ClC-2 Cl(-) channels. Reverse transcription polymerase chain reaction and Northern blot analysis confirmed transcriptional expression of ClC-2 in both atrial and ventricular tissues and isolated myocytes of mouse and guinea pig hearts. These results indicate that a novel I(Cl.ir) is present in mammalian heart and support a potentially important role of ClC-2 channels in the regulation of cardiac electrical activity and cell volume under physiological and pathological conditions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aspartic Acid / pharmacology
  • Blotting, Northern
  • Cadmium / pharmacology
  • Chloride Channels / antagonists & inhibitors
  • Chloride Channels / drug effects
  • Chloride Channels / genetics
  • Chloride Channels / metabolism*
  • Guinea Pigs
  • Heart Atria / metabolism
  • Heart Ventricles / metabolism
  • In Vitro Techniques
  • Meglumine / pharmacology
  • Mice
  • Myocardium / metabolism*
  • Patch-Clamp Techniques
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Chloride Channels
  • ClC-2 chloride channels
  • Cadmium
  • Aspartic Acid
  • RNA
  • Meglumine