Nonaqueous versus aqueous capillary electrophoresis for the dosage of N-butylscopolamine in various pharmaceutical formulations

J Pharm Biomed Anal. 1999 Oct;21(1):165-74. doi: 10.1016/s0731-7085(99)00141-7.


A simple nonaqueous capillary electrophoresis method is described for the separation of several atropine and scopolamine related drugs. The analysis of these pharmaceutical compounds was performed in a methanol-acetonitrile (25/5, v/v) mixture containing 25 mM ammonium acetate and 1 M acetic acid. The robustness was proved using a full factorial design at two levels. The method was validated and successfully applied for the determination of N-butylscopolamine in different pharmaceutical preparations. Results were compared to those obtained by a capillary electrophoresis method based on aqueous media.

MeSH terms

  • Butylscopolammonium Bromide / analysis*
  • Chemistry, Pharmaceutical
  • Electrophoresis, Capillary / methods*
  • Muscarinic Antagonists / analysis
  • Quality Control
  • Reproducibility of Results


  • Muscarinic Antagonists
  • Butylscopolammonium Bromide