Abstract
The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens of Leishmania braziliensis. The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Animals
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Antibodies, Protozoan / blood*
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Antigens, Protozoan / immunology*
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Child
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Child, Preschool
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Female
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Humans
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Immunoblotting / methods
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Leishmania braziliensis / immunology*
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Leishmaniasis, Cutaneous / blood
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Leishmaniasis, Cutaneous / diagnosis
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Leishmaniasis, Cutaneous / immunology*
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Male
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Middle Aged
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Sensitivity and Specificity
Substances
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Antibodies, Protozoan
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Antigens, Protozoan