Microvascular repair following a modified crush-avulsion injury in a rat model: effect of recombinant human tissue-type plasminogen activator on the patency rate

Microsurgery. 2000;20(2):52-8. doi: 10.1002/(sici)1098-2752(2000)20:2<52::aid-micr2>3.0.co;2-g.


The failure rate of replantations following a crush-avulsion type injury is high. This study has been designed to reproduce an effective standardized crush-avulsion injury model to the femoral artery of the rat and evaluate the antithrombotic efficacy of systemic intravenous administration of recombinant human tissue-type plasminogen activator (rt-PA). The crush-avulsion injury was reproduced by using a bulldog clamp and two hemostats and followed by microvascular repair. The animals were divided into three groups of 20 rats each and received either normal saline, heparin 100 U/kg body weight, or rt-PA 3.5 mg/kg body weight intravenously. Patency tests were performed 20 min and 48 h after blood flow reestablishment. Results showed that this experimental crush-avulsion injury model ensures low patency in the control group, whereas systemic rt-PA administration improves the patency rate statistically significantly compared to control and heparin groups at both 20 min and 48 h postrevascularization.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Evaluation Studies as Topic
  • Femoral Artery / injuries
  • Fibrinolytic Agents / pharmacology*
  • Humans
  • Male
  • Microsurgery*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Recombinant Proteins
  • Tissue Plasminogen Activator / pharmacology*
  • Vascular Patency / drug effects*
  • Vascular Surgical Procedures*


  • Fibrinolytic Agents
  • Recombinant Proteins
  • Tissue Plasminogen Activator