Flow injection analysis of mercury(II) in pharmaceuticals based on enzyme inhibition and biosensor detection

J Pharm Biomed Anal. 1999 Jun;20(1-2):263-9. doi: 10.1016/s0731-7085(99)00032-1.

Abstract

An enzymatic amperometric procedure for measurement of mercury(II) in pharmaceuticals, based on the inhibition of invertase and on a glucose electrode was studied. Analytical parameters for measurements in batch and flow injection analysis (FIA) have been optimised. Mercury(II) was detected in the 10-60 ppb range with RSD < or =2%. A sample throughput of 6 h(-1) for batch and 15 h(-1) for FIA was obtained. The total mercury(II) from thimerosal (thiomersal, sodium ethylmercurithiosalicylate) in eye-drop samples was measured with the amperometric procedure after oxidative cleavage treatment. Results for both batch and FIA procedures correlated well with atomic absorbtion spectroscopy (AAS) data.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • Enzyme Inhibitors / pharmacology*
  • Enzymes, Immobilized
  • Flow Injection Analysis
  • Glucose / analysis
  • Glycoside Hydrolases / antagonists & inhibitors*
  • Indicators and Reagents
  • Mercury / analysis*
  • Mercury / pharmacology
  • Spectrophotometry, Atomic
  • Sucrose / analysis
  • beta-Fructofuranosidase

Substances

  • Enzyme Inhibitors
  • Enzymes, Immobilized
  • Indicators and Reagents
  • Sucrose
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • Mercury
  • Glucose