Expression of Cancer-Testis Antigens in Lung Cancer: Definition of Bromodomain Testis-Specific Gene (BRDT) as a New CT Gene, CT9

Cancer Lett. 2000 Mar 31;150(2):155-64. doi: 10.1016/s0304-3835(99)00385-7.

Abstract

In an effort to define new cancer-testis (CT) genes, we investigated whether BRDT, a testis-restricted member of the RING3 family of transcriptional regulators, is also expressed in cancer. Standard RT-PCR expression analysis detected BRDT transcripts in 12 of 47 cases of non-small cell lung cancer and single cases of both squamous cell carcinoma of the head and neck (1/12) and esophagus (1/12) but not in melanoma or in cancers of the colon, breast, kidney and bladder. Typing of 33 non-small cell lung cancers for coexpression of a panel of CT antigens revealed a high incidence (60%) of MAGE-3 mRNA expression, followed by MAGE-1 (36%), CT7/MAGE-C1 (30%), CT10 (30%), SSX4 (23%), BRDT (21%), NY-ESO-1 (21%) and HOM-MEL-40/SSX2 (15%). The coexpression pattern of these antigens provides a foundation for developing a polyvalent lung cancer vaccine.

MeSH terms

  • Antigens, Neoplasm / genetics*
  • Cancer Vaccines / immunology
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Humans
  • Lung Neoplasms / metabolism*
  • Male
  • Nuclear Proteins / genetics*
  • RNA, Messenger / analysis*
  • Testis / metabolism*
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • BRDT protein, human
  • Cancer Vaccines
  • Nuclear Proteins
  • RNA, Messenger