BMP-4 affects the differentiation of metanephric mesenchyme and reveals an early anterior-posterior axis of the embryonic kidney

Dev Dyn. 2000 Feb;217(2):146-58. doi: 10.1002/(SICI)1097-0177(200002)217:2<146::AID-DVDY2>3.0.CO;2-I.

Abstract

Bone morphogenetic protein-4 (BMP4), a member of the transforming growth factor-beta (TGF-beta) family, regulates several developmental processes during animal development. We have now studied the effects of BMP-4 in the metanephric kidney differentiation by using organ culture technique. Human recombinant BMP-4 diminishes the number of ureteric branches and changes the branching pattern. Our data suggest that BMP-4 affects the ureteric branching indirectly via interfering with the differentiation of the nephrogenic mesenchyme. The clear positional preference of the defects to posterior mesenchyme might reflect an early anterior-posterior patterning of the metanephric mesenchyme. The smooth muscle alpha-actin expressing cell population around the ureteric stalk, highly expressing Bmp-4 mRNA, is also expanded in kidneys treated with BMP-4. Thus, BMP-4 may be a physiological regulator of the development of the periureteric smooth muscle layer and ureteric elongation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axis, Cervical Vertebra
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins / pharmacology*
  • Humans
  • Kidney / drug effects*
  • Kidney / embryology*
  • Mesoderm / drug effects
  • Mice
  • Mice, Inbred CBA
  • Organ Culture Techniques
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / pharmacology

Substances

  • BMP4 protein, human
  • Bmp4 protein, mouse
  • Bmp4 protein, rat
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins
  • Recombinant Proteins