Human herpesvirus 8 and Epstein Barr-virus in a cutaneous B-cell lymphoma and a malignant cell line established from the blood of an AIDS patient

Leuk Lymphoma. 1999 Oct;35(3-4):379-87. doi: 10.3109/10428199909145743.


Human Herpesvirus 8 (HHV-8) has been consistently associated with Primary Effusion Lymphoma (PEL or body-cavity-based lymphoma) but not with other lymphomas. This paper reports on an AIDS patient without obvious malignant effusion in body cavities but with a cutaneous lymphoma where HHV-8 and Epstein-Barr virus (EBV) were detected by PCR and electron microscopy. Both viruses were also detected in all the cells of a malignant cell line (BBG1) established from the patient's peripheral blood mononuclear cells. As in PEL and PEL-derived cell lines, both the tumor and the lines lacked B-antigen expression in immunological studies but were of the same B origin as shown by clonal immunoglobulin gene rearrangements. In contrast to other co-infected cell lines, BBG1 and subclones spontaneously expressed the HHV-8 lytic antigens p40, p27, p60 and the EBV transforming latent antigen EBNA2. These data suggest that the clinical and biological features of HHV-8-and EBV-associated lymphomas could be wider than has been described to date in PEL particularly with the in vivo presence of circulating malignant dually-infected cells engaged in a spontaneous HHV-8 lytic infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / blood
  • Acquired Immunodeficiency Syndrome / complications
  • Acquired Immunodeficiency Syndrome / pathology
  • Acquired Immunodeficiency Syndrome / virology*
  • Herpesvirus 4, Human / isolation & purification*
  • Herpesvirus 8, Human / isolation & purification*
  • Humans
  • Lymphoma / etiology
  • Lymphoma / virology*
  • Lymphoma, B-Cell / etiology
  • Lymphoma, B-Cell / virology*
  • Molecular Sequence Data
  • Skin Neoplasms / etiology
  • Skin Neoplasms / virology*
  • Tumor Cells, Cultured

Associated data

  • GENBANK/AF042370
  • GENBANK/AF042371
  • GENBANK/AF042372