Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

Vaccine. 2000 Apr 3;18(19):2015-22. doi: 10.1016/s0264-410x(99)00528-9.

Abstract

We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8(+) T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / blood
  • Antigens, CD / genetics
  • Antigens, CD / immunology
  • Antigens, Viral / genetics
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Female
  • Humans
  • Immunization, Secondary
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, Inbred C57BL
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / genetics
  • Papillomaviridae / immunology*
  • Papillomavirus E7 Proteins
  • Papillomavirus Infections / immunology
  • Papillomavirus Infections / prevention & control
  • Papillomavirus Vaccines*
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / prevention & control
  • Uterine Cervical Neoplasms / immunology
  • Uterine Cervical Neoplasms / prevention & control
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / genetics
  • Vaccinia virus / genetics
  • Vaccinia virus / immunology*
  • Viral Vaccines / administration & dosage*
  • Viral Vaccines / genetics

Substances

  • Antibodies, Viral
  • Antigens, CD
  • Antigens, Viral
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Glycoproteins
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • Papillomavirus Vaccines
  • Vaccines, DNA
  • Viral Vaccines
  • oncogene protein E7, Human papillomavirus type 16