Neuropilin-2 Regulates the Development of Selective Cranial and Sensory Nerves and Hippocampal Mossy Fiber Projections

Neuron. 2000 Jan;25(1):43-56. doi: 10.1016/s0896-6273(00)80870-3.

Abstract

Neuropilin-1 and neuropilin-2 bind differentially to different class 3 semaphorins and are thought to provide the ligand-binding moieties in receptor complexes mediating repulsive responses to these semaphorins. Here, we have studied the function of neuropilin-2 through analysis of a neuropilin-2 mutant mouse, which is viable and fertile. Repulsive responses of sympathetic and hippocampal neurons to Sema3F but not to Sema3A are abolished in the mutant. Marked defects are observed in the development of several cranial nerves, in the initial central projections of spinal sensory axons, and in the anterior commissure, habenulo-interpeduncular tract, and the projections of hippocampal mossyfiber axons in the infrapyramidal bundle. Our results show that neuropilin-2 is an essential component of the Sema3F receptor and identify key roles for neuropilin-2 in axon guidance in the PNS and CNS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axons / physiology
  • COS Cells
  • Cranial Nerves / chemistry
  • Cranial Nerves / embryology*
  • Cranial Nerves / pathology
  • Gene Expression Regulation, Developmental / physiology
  • Genes, Reporter
  • Glycoproteins / physiology
  • Habenula / chemistry
  • Habenula / embryology
  • Habenula / pathology
  • Mice
  • Mice, Knockout
  • Mossy Fibers, Hippocampal / chemistry
  • Mossy Fibers, Hippocampal / embryology*
  • Mossy Fibers, Hippocampal / pathology
  • Mutagenesis / physiology
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism*
  • Neurons, Afferent / chemistry*
  • Neurons, Afferent / physiology
  • Neurons, Afferent / ultrastructure
  • Neuropilin-1
  • Peripheral Nerves / chemistry
  • Peripheral Nerves / cytology
  • Peripheral Nerves / embryology
  • Semaphorin-3A
  • Spinal Nerve Roots / chemistry
  • Spinal Nerve Roots / cytology
  • Spinal Nerve Roots / embryology
  • Superior Cervical Ganglion / chemistry
  • Superior Cervical Ganglion / embryology
  • Superior Cervical Ganglion / pathology
  • beta-Galactosidase / genetics

Substances

  • Glycoproteins
  • Nerve Tissue Proteins
  • Semaphorin-3A
  • Neuropilin-1
  • beta-Galactosidase