Preterm delivery in mice with renal abscess

Obstet Gynecol. 2000 Mar;95(3):453-6. doi: 10.1016/s0029-7844(99)00571-2.

Abstract

Objective: Our purpose was to develop a mouse model of renal abscess to study the effect of extrauterine infection on preterm delivery.

Methods: Escherichia coli or sterile medium was injected into the left kidney of 70 pregnant mice that had completed approximately 75% of gestation. Preterm delivery rates were recorded for various inocula. Kidney specimens were obtained and examined grossly and histologically for abscess formation.

Results: Thirty-one of 51 animals (60.8%) infected with 1 x 10(5)-9 x 10(6) bacteria and none of 19 uninfected animals delivered prematurely (P < .001). Renal abscess was induced in 100% of mice receiving bacterial inoculation but in none receiving sterile medium.

Conclusion: Kidney injection provides a reliable method for inducing renal abscess in pregnant mice. Renal abscess induces preterm delivery at a stable rate across a wide range of bacterial inocula. This model of extrauterine infection may be particularly useful in investigations of infection-induced preterm delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscess / complications*
  • Animals
  • Disease Models, Animal*
  • Female
  • Kidney Diseases / complications*
  • Mice
  • Mice, Inbred Strains
  • Obstetric Labor, Premature / etiology*
  • Pregnancy