Genomic-scale analysis of gene expression in resting and activated T cells

Curr Opin Immunol. 2000 Apr;12(2):206-9. doi: 10.1016/s0952-7915(99)00075-8.

Abstract

Recent advances in gene array technology and isolation of lymphocytes now allow comprehensive analysis of gene expression in many different types of T cells. So far only a few sets of results have been published. However it is already clear that these analyses provide accurate measurements of gene expression in T cells. This technology offers the first opportunity to examine global and subtle changes in gene expression in response to specific stimuli.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Cell Separation
  • Databases, Factual
  • Expressed Sequence Tags
  • Gene Expression Profiling*
  • Gene Expression Regulation / drug effects
  • Genome*
  • Hot Temperature
  • Humans
  • Jurkat Cells
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / genetics*
  • Mice
  • Oligonucleotide Array Sequence Analysis*
  • Phorbol Esters / pharmacology
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism

Substances

  • Phorbol Esters
  • RNA, Messenger