Characterization and chromosomal mapping of a human Necdin pseudogene

Gene. 2000 Mar 7;245(1):185-91. doi: 10.1016/s0378-1119(00)00012-3.


The necdin gene is expressed predominantly in postmitotic neurons and encodes a growth suppressor that interacts with the transcription factors E2F1 and p53. Human necdin gene (NDN) is maternally imprinted and located in Prader-Willi syndrome deletion region 15q11.2-q12. We isolated an NDN homologous sequence from a human genomic DNA library. The homologous sequence is overall 83% identical with necdin cDNA sequence, and possesses a short poly(A) stretch at the 3' end and direct repeats at both ends. Expression of the homologous sequence, which lacks a 5' promoter sequence, was undetected in cultured human cell lines. We mapped this sequence to chromosome 12q14-q21.1 by fluorescence in situ hybridization. These characteristics of the NDN-homologous sequence are consistent with those of processed pseudogenes. The information about the necdin pseudogene in the human genome will be useful for genetic studies on NDN-associated neurogenic disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • Cell Adhesion Molecules / genetics*
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12 / genetics
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / genetics
  • DNA / isolation & purification
  • Gene Expression Regulation, Neoplastic
  • Glioma / genetics
  • Glioma / pathology
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Nectins
  • Neuroblastoma / genetics
  • Neuroblastoma / pathology
  • Pseudogenes / genetics*
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Tumor Cells, Cultured


  • Cell Adhesion Molecules
  • Nectins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/AB028239