Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells

Oncogene. 2000 Mar 2;19(10):1266-76. doi: 10.1038/sj.onc.1203433.


We have previously shown that the transcription factor AP-1 regulates the expression of genes which allow neoplastically transformed rat fibroblasts to become invasive. Searches for further AP-1 target genes led to the identification of a gene encoding a novel rat kelch family member, named kelch related protein 1 (Krp1). Kelch family members are characterized by a series of repeats at their carboxyl terminus and a BTB/POZ domain near their amino terminus. Rat Krp1 has a primarily cytoplasmic localization, and a small fraction appears to accumulate and co-localize with F-actin at membrane ruffle-like structures in the tips of pseudopodia. Overexpression of Krp1 in transformed rat fibroblasts led to the formation of dramatically elongated pseudopodia, while expression of truncated Krp1 polypeptides resulted in a reduction in the length of pseudopodia. We propose that the transformation-specific expression of Krp1 is required for pseudopod elongation, which are structures that are required for cell motility and invasion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / isolation & purification
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification*
  • Cell Transformation, Neoplastic*
  • Cytoskeletal Proteins*
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Oncogene Proteins v-fos / genetics
  • Pseudopodia / physiology*
  • Rats
  • Sequence Homology, Amino Acid
  • Up-Regulation
  • ras Proteins / genetics


  • Actins
  • Carrier Proteins
  • Cytoskeletal Proteins
  • KLHL41 protein, human
  • Klhl41 protein, rat
  • Oncogene Proteins v-fos
  • ras Proteins