Antiinflammatory effects of seaprose-S on various inflammation models

Drugs Exp Clin Res. 1999;25(6):263-70.


The antiinflammatory activity of seaprose-S in different experimental models involving different biochemical mediators of inflammation was investigated. In vivo experiments were performed using male Sprague-Dawley rats and in vitro experiments were performed using articular cartilage explants of pig joints. In acute experimental models of inflammation, 0.5, 1 or 2 mg/kg of seaprose-S was injected intravenously (i.v.) before challenge with inflammatory agents. In adjuvant-induced arthritis, seaprose-S was given as a 2 mg/kg i.v. dose once a day for 4 consecutive days from day 8 after injection of the adjuvant. In cartilage-synovium cocultures, seaprose-S was incubated at a concentration of 0.001 microM and 0.05 microM. Paw volume was measured with a plethysmograph and proteoglycan synthesis was determined in articular cartilage-synovium coculture by incorporation of 35S-sulfate. Seaprose-S inhibited inflammation dose-dependently in carrageenan, concanavalin-A, FeCl2, nystatin-induced paw edema and in carrageenan-induced pleurisy and acetic acid-induced peritonitis. In Freund's adjuvant-induced arthritis, seaprose-S significantly reduced the primary and secondary lesions. In vitro on articular cartilage, seaprose-S increased proteoglycan synthesis in the cartilage alone and reduced the inhibition of proteoglycan synthesis in the cartilage cocultured with minced synovium.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Arthritis, Experimental / drug therapy
  • Cartilage, Articular / drug effects
  • Cartilage, Articular / metabolism
  • Disease Models, Animal
  • Edema / drug therapy
  • Hydrocortisone / pharmacology
  • In Vitro Techniques
  • Indomethacin / pharmacology
  • Inflammation / drug therapy*
  • Male
  • Proteoglycans / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Serine Endopeptidases / pharmacology*
  • Swine
  • Synovial Membrane / drug effects
  • Synovial Membrane / metabolism


  • Anti-Inflammatory Agents
  • Anti-Inflammatory Agents, Non-Steroidal
  • Proteoglycans
  • Seaprose S
  • Serine Endopeptidases
  • Hydrocortisone
  • Indomethacin