Cloning and characterization of prostaglandin endoperoxide synthase-1 and -2 from the brook trout ovary

Mol Cell Endocrinol. 2000 Feb 25;160(1-2):89-97. doi: 10.1016/s0303-7207(99)00252-x.

Abstract

Using a combination of reverse transcription-PCR and library screening, the cDNAs for prostaglandin endoperoxide synthase-1 (PGS-1) and 2 (PGS-2) were isolated from the brook trout ovary. The brook trout PGS-1 cDNA encodes for a 598 amino acid protein that is 69% identical to mammalian PGS-1. PGS-1 transcripts were observed in the ovary, spleen, gills, head kidney, trunk kidney, intestine, stomach, skin and heart. To our knowledge, this is the first characterization of a non-mammalian PGS-1 cDNA. The brook trout PGS-2 encodes for a 607 amino acid protein that is 69% identical to mammalian PGS-2 and was observed in the skin, gills, stomach and heart. PGS-2 transcripts were highly upregulated in the ovaries by the phorbol ester, phorbol-12-myristate-13-acetate, in combination with the calcium ionophore, A23187. However, PGS-2 was not observed in the ovary of brook trout undergoing natural oocyte maturation and ovulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Female
  • Isoenzymes / genetics*
  • Mammals
  • Molecular Sequence Data
  • Ovary / enzymology*
  • Prostaglandin-Endoperoxide Synthases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Tissue Distribution
  • Trout / genetics*
  • Trout / metabolism*

Substances

  • DNA Primers
  • DNA, Complementary
  • Isoenzymes
  • RNA, Messenger
  • Prostaglandin-Endoperoxide Synthases