Disinfection potential of electrolyzed solutions containing sodium chloride at low concentrations

J Virol Methods. 2000 Mar;85(1-2):163-74. doi: 10.1016/s0166-0934(99)00165-2.


Electrolyzed products of sodium chloride solution were examined for their disinfection potential against hepatitis B virus (HBV) and human immunodeficiency virus (HIV) in vitro. Electrolysis of 0.05% NaCl in tap water was carried out for 45 min at room temperature using a 3 A electric current in separate wells installed with positive and negative electrodes. The electrolyzed products were obtained from the positive well. The oxidation reduction potential (ORP), pH and free chlorine content of the product were 1053 mV, pH 2.34 and 4.20 ppm, respectively. The products modified the antigenicity of the surface protein of HBV as well as the infectivity of HIV in time- and concentration-dependent manner. Although the inactivating potential was decreased by the addition of contaminating protein, recycling of the product or continuous addition of fresh product may restore the complete disinfection against bloodborne pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids
  • Attention
  • Cell Line
  • Disinfection / methods*
  • Electrolysis
  • HIV-1*
  • Hepatitis B virus*
  • Humans
  • Hydrogen-Ion Concentration
  • Oxidation-Reduction
  • Sodium Chloride* / chemistry
  • Solutions


  • Acids
  • Solutions
  • Sodium Chloride