Abstract
We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism*
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Bacterial Proteins*
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Cell Division / genetics
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DNA, Bacterial / chemistry*
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DNA, Bacterial / metabolism*
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Ribosomal Proteins / metabolism*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Hbsu protein, bacteria
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Recombinant Proteins
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Ribosomal Proteins
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ribosomal protein L17