The L17 ribosomal protein of Bacillus subtilis binds preferentially to curved DNA

Biochimie. 2000 Jan;82(1):85-91. doi: 10.1016/s0300-9084(00)00184-x.

Abstract

We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism*
  • Bacterial Proteins*
  • Cell Division / genetics
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / metabolism*
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Ribosomal Proteins / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Hbsu protein, bacteria
  • Recombinant Proteins
  • Ribosomal Proteins
  • ribosomal protein L17