Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary

J Neuroendocrinol. 2000 Mar;12(3):263-72. doi: 10.1046/j.1365-2826.2000.00447.x.

Abstract

The expression of mRNA for voltage-dependent (Kv) and inward-rectifying K channels (Kir) was studied in clonal rat somato-mammotroph cells (GH3/B6 cells) and rat pituitary using reverse transcription-polymerase chain reaction (RT-PCR). In GH3/B6 cells transcripts for 16 different Kv channel alpha-subunits (seven Shaker-related: Kv1.2, Kv1.4, Kv1.5, Kv2.1, Kv3.2, Kv4.1, Kv5.1; six EAG: eag1, erg1, erg2, elk1-elk3; three KCNQ: KCNQ1-KCNQ3) and for five different Kir channel alpha-subunits (Kir1.1, Kir2.3, Kir3.2, Kir3.3, Kir6.2) were found. In addition, transcripts for a short isoform of Kvbeta2 and transcripts for Kvbeta3 subunits were present. In rat pituitary transcripts for 21 different Kv channel alpha-subunits (11 Shaker-related: Kv1.3, Kv1.4, Kv1.6, Kv2.1, Kv2.2, Kv3.2, Kv3.4, Kv4.1, Kv4.2, Kv4.3, Kv6.1; seven EAG: eag1, erg1-erg3, elk1-elk3; three KCNQ: KCNQ1-KCNQ3) and nine Kir channel alpha-subunits (Kir1.1, Kir2.2, Kir3.1-Kir3.4, Kir4.1, Kir6.1, Kir6. 2) were found. In addition, all tested auxiliary subunits (Kvbeta1-Kvbeta3, minK, SUR1, SUR2) are expressed in the pituitary. The results indicate that the macroscopic K currents in GH3/B6 and pituitary cells are presumably mediated by K channels constructed by a larger number of K channel alpha-subunits and auxiliary beta-subunits than previously distinguished electrophysiologically and pharmacologically.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Electric Conductivity
  • Ether-A-Go-Go Potassium Channels
  • Female
  • Gene Expression*
  • Growth Hormone / analysis
  • KCNQ Potassium Channels
  • KCNQ1 Potassium Channel
  • Pituitary Gland / chemistry*
  • Potassium Channels / genetics*
  • Potassium Channels, Voltage-Gated*
  • Prolactin / analysis
  • RNA, Messenger / analysis*
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Shaker Superfamily of Potassium Channels

Substances

  • Ether-A-Go-Go Potassium Channels
  • KCNQ Potassium Channels
  • KCNQ1 Potassium Channel
  • Kcnh1 protein, rat
  • Kcnq1 protein, rat
  • Potassium Channels
  • Potassium Channels, Voltage-Gated
  • RNA, Messenger
  • Shaker Superfamily of Potassium Channels
  • Prolactin
  • Growth Hormone