Development of a novel biomarker of free radical damage in reperfusion injury after cardiac arrest

FEBS Lett. 2000 Mar 17;470(1):1-6. doi: 10.1016/s0014-5793(00)01279-5.


In a porcine model of cardiopulmonary resuscitation (CPR), we investigated changes in the plasma levels of 8-iso-PGF(2alpha), a marker for oxidative injury, and 15-keto-dihydro-PGF(2alpha), an inflammatory response indicator during the post-resuscitation period after cardiac arrest. Twelve piglets were subjected to either 2 or 5 min (VF2 and VF5 group) of ventricular fibrillation (VF) followed by 5 min of closed-chest CPR. Six piglets without cardiac arrest were used as controls. In VF5 group, 8-iso-PGF(2alpha) in the jugular bulb plasma (draining the brain) increased four-fold. Jugular bulb 8-iso-PGF(2alpha) in the control group remained unchanged. The 15-keto-dihydro-PGF(2alpha) also increased four-fold in the VF5 group. Thus, 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha) measurements in jugular bulb plasma may be used as biomarkers for quantification of free radical catalyzed oxidative brain injury and inflammatory response in reperfusion injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Cardiopulmonary Resuscitation
  • Dinoprost / analogs & derivatives*
  • Dinoprost / blood
  • Dinoprost / chemistry
  • Disease Models, Animal
  • F2-Isoprostanes
  • Female
  • Free Radicals
  • Heart Arrest / blood*
  • Heart Arrest / complications
  • Jugular Veins
  • Male
  • Molecular Structure
  • Myocardial Reperfusion Injury / blood*
  • Swine
  • Ventricular Fibrillation


  • Biomarkers
  • F2-Isoprostanes
  • Free Radicals
  • 15-keto-13,14-dihydroprostaglandin F2alpha
  • 8-epi-prostaglandin F2alpha
  • Dinoprost