Hematopoietic reconstitution after lethal irradiation and bone marrow transplantation: effects of different hematopoietic cytokines on the recovery of thymus, spleen and blood cells

Bone Marrow Transplant. 2000 Feb;25(4):427-33. doi: 10.1038/sj.bmt.1702169.


Lethally irradiated mice were grafted with syngeneic bone marrow cells or left ungrafted. Mice of each group were injected with different hematopoietic cytokines for 5 consecutive days starting immediately after irradiation or left uninjected. The recovery of lymphoid tissues induced by hematopoietic cytokines 7 days after irradiation and bone marrow cell transplantation was comparable to that observed at days 21-28 in irradiated, bone marrow-grafted, but cytokine-uninjected mice. IL-11 or IL-6, in combination with IL-3, was able to hasten thymus, spleen and blood cell numbers and functions. SCF also displayed a detectable effect when used with IL-3. Conversely, the IL-6 superagonist K-7/D-6 was able, when injected alone, to induce significant recovery of thymus, spleen and blood cells. Thus, K-7/D-6 appears to be a most efficient cytokine for fast reconstitution of lymphoid tissues after irradiation and bone marrow transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Cells / pathology
  • Blood Cells / physiology
  • Bone Marrow Transplantation*
  • Female
  • Graft Survival / drug effects
  • Hematopoiesis*
  • Interleukin-11 / pharmacology*
  • Interleukin-3 / pharmacology*
  • Interleukin-6 / pharmacology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Spleen / physiopathology
  • Thymus Gland / physiopathology
  • Transplantation, Isogeneic
  • Whole-Body Irradiation


  • Interleukin-11
  • Interleukin-3
  • Interleukin-6