Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus

Phytochemistry. 2000 Mar;53(5):549-53. doi: 10.1016/s0031-9422(99)00582-8.


Precursor administration experiments with 2H-labeled 6-oxocampestanol, 6-deoxocastasterone and 6alpha-hydroxycastasterone in cultured cells of Catharanthus roseus were performed and the metabolites were analyzed by GC-MS. [2H6]Cathasterone was identified as a metabolite of [2H6]6-oxocampestanol, whereas [2H6]6alpha-hydroxycastasterone and [2H6]castasterone were identified as metabolites of [2H6]6-deoxocastasterone, and [2H6]castasterone was identified as a metabolite of [2H6]6alpha-hydroxycastasterone, indicating that 6-deoxocastasterone is converted to castasterone via 6alpha-hydroxycastasterone. In addition, 6-deoxocathasterone, a putative biosynthetic intermediate in the late C6-oxidation pathway, was identified as an endogenous brassinosteroid. These studies provide further evidence supporting our proposed biosynthetic pathways for brassinolide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cholestanols / chemistry
  • Cholestanols / metabolism
  • Phytosterols / metabolism
  • Plants / chemistry
  • Plants / metabolism*
  • Steroids / biosynthesis*


  • 6-deoxacastasterone
  • 6-oxocampestanol
  • Cholestanols
  • Phytosterols
  • Steroids
  • castasterone