Antioxidant properties of a North American ginseng extract

Mol Cell Biochem. 2000 Jan;203(1-2):1-10. doi: 10.1023/a:1007078414639.

Abstract

A North American ginseng extract (NAGE) containing known principle ginsenosides for Panax quinquefolius was assayed for metal chelation, affinity to scavenge DPPH-stable free radical, and peroxyl (LOO*) and hydroxyl (*OH) free radicals for the purpose of characterizing mechanisms of antioxidant activity. Dissociation constants (Kd) for NAGE to bind transition metals were in the order of Fe2+ > Cu2+ > Fe3+ and corresponded to the affinity to inhibit metal induced lipid peroxidation. In a metal-free linoleic acid emulsion, NAGE exhibited a significant (p < or = 0.05) concentration (0.01-10 mg/mL) dependent mitigation of lipid oxidation as assessed by the ammonium thiocyanate method. Similar results were obtained when NAGE was incubated in a methyl linoleate emulsion containing haemoglobin catalyst and assessed by an oxygen electrode. NAGE also showed strong DPPH radical scavenging activity up to a concentration of 1.6 mg/mL (r2 = 0.996). Similar results were obtained for scavenging of both site-specific and non site-specific *OH, using the deoxyribose assay method. Moreover, NAGE effectively inhibited the non site-specific DNA strand breakage caused by Fenton agents, and suppressed the Fenton induced oxidation of a 66 Kd soluble protein obtained from mouse brain over a concentration range of 2-40 mg/mL. These results indicate that NAGE exhibits effective antioxidant activity in both lipid and aqueous mediums by both chelation of metal ions and scavenging of free radicals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Brain / drug effects
  • Brain / metabolism
  • Catalysis
  • Free Radical Scavengers / pharmacology
  • Hemoglobins / metabolism
  • Lipid Peroxidation / drug effects
  • Metals / metabolism
  • Mice
  • Panax / chemistry*
  • Plant Extracts / pharmacology*
  • Plants, Medicinal*
  • Rats
  • Thiocyanates / metabolism

Substances

  • Antioxidants
  • Free Radical Scavengers
  • Hemoglobins
  • Metals
  • Plant Extracts
  • Thiocyanates
  • thiocyanic acid