Glycoprotein G of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies

J Gen Virol. 2000 Apr;81(Pt 4):1033-40. doi: 10.1099/0022-1317-81-4-1033.

Abstract

Serological diagnosis of herpes simplex virus (HSV) infections requires assays based on antigens that expose type-specific determinants. This study was designed to outline the B-cell epitopes of the type-specific glycoprotein G-1 (gG-1) of HSV type 1 (HSV-1), by investigating the reactivity of human anti-gG-1 antibodies, purified from 21 HSV-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gG-1 sequence. The epitope mapping demonstrated that these antibodies bound preferentially to antigenic determinants that localized to regions with a high degree of amino acid similarity to the corresponding glycoprotein in HSV-2, gG-2. In spite of this, the purified anti-gG-1 antibodies were found to be non-reactive to native gG-2 antigen, as well as to overlapping gG-2 peptides, thus supporting the role of gG-1 as a prototype HSV-1 type-specific antigen. One immunodominant region, delimited by amino acids 112-127, reacted with all purified anti-gG-1 antibodies and may be of interest for the further development of a peptide-based HSV-1 type-specific seroassay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies / immunology
  • Antibody Specificity
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology*
  • Epitope Mapping
  • Epitopes / genetics
  • Epitopes / immunology*
  • Herpesvirus 1, Human / immunology*
  • Humans
  • Molecular Sequence Data
  • Sequence Alignment
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies
  • Antigens, Viral
  • Epitopes
  • Viral Envelope Proteins
  • glycoprotein gG-1, herpes simplex virus type 1