Mutation in bombesin receptor subtype-3 gene is not a major cause of obesity in the Japanese

Horm Metab Res. 2000 Jan;32(1):33-4. doi: 10.1055/s-2007-978582.


Bombesin receptor subtype-3 (BRS-3) is one of the candidate genes of obesity. The mice lacking BRS-3 have been shown to develop mild obesity. These mice also showed hypertension and impaired glucose metabolism, supporting these mice as a good model for human obesity. We screened 104 Japanese obese men (BMI > 26.4, 26.5-44.1) to investigate whether there is any genetic defect in BRS-3 gene. The DNA fragments containing each exon of BRS-3 gene were amplified by polymerase chain reaction (PCR) and were directly sequenced. No mutation, nor polymorphism was found in the coding region of BRS-3, suggesting that mutation of this gene is not a major cause of obesity in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • Genetic Testing
  • Humans
  • Japan
  • Mutation
  • Obesity / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Receptors, Bombesin / genetics*
  • Sequence Analysis, DNA


  • DNA Primers
  • Receptors, Bombesin
  • bombesin receptor subtype 3