Analysis of Drosophila salivary gland, epidermis and CNS development suggests an additional function of brinker in anterior-posterior cell fate specification

Mech Dev. 2000 Apr;92(2):179-91. doi: 10.1016/s0925-4773(99)00337-8.

Abstract

Salivary glands are simple structured organs which can serve as a model system in the study of organogenesis. Following a large EMS mutagenesis we have identified a number of genes required for normal salivary gland development. Mutations in the locus small salivary glands-1 (ssg-1) lead to a drastic reduction in the size of the salivary glands. The gene ssg-1 was cloned and subsequent sequence and genetic analysis showed identity to the recently published gene brinker. The salivary gland placode in brinker mutants appears reduced along both the anterior-posterior and dorso-ventral axis. Analysis of the brinker cuticle phenotype revealed a similar loss of anterior-posterior as well as lateral cell fates. The abdominal ventral denticle belts show a reduced number of setae in the first denticle row. Furthermore, we observed a preferential loss of lateral neuroblasts in the anterior parasegment. Together, these phenotypes suggest that brinker not only plays a role in dorso-ventral but also in anterior-posterior axis patterning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial*
  • Animals
  • Bacterial Proteins / metabolism
  • Body Patterning / genetics
  • Cell Differentiation / genetics
  • Central Nervous System / cytology
  • Central Nervous System / embryology*
  • Chromosome Mapping
  • Drosophila / embryology*
  • Drosophila / genetics
  • Drosophila Proteins*
  • Embryo, Nonmammalian
  • Embryonic Induction / genetics
  • Epidermal Cells
  • Epidermis / embryology*
  • Gene Expression Regulation, Developmental
  • Genetic Complementation Test
  • Homeodomain Proteins / metabolism
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism
  • Mutation
  • Neuropeptides / metabolism
  • Receptors, Steroid / metabolism
  • Repressor Proteins*
  • Salivary Glands / abnormalities
  • Salivary Glands / cytology
  • Salivary Glands / embryology*
  • Transcription Factors*

Substances

  • Adhesins, Bacterial
  • Bacterial Proteins
  • Drosophila Proteins
  • Homeodomain Proteins
  • Insect Proteins
  • Neuropeptides
  • Receptors, Steroid
  • Repressor Proteins
  • Transcription Factors
  • brk protein, Drosophila
  • dpp protein, Drosophila
  • eg protein, Drosophila
  • eve protein, Drosophila
  • invasin, Yersinia