The genome sequence of Drosophila melanogaster

Science. 2000 Mar 24;287(5461):2185-95. doi: 10.1126/science.287.5461.2185.


The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. Efforts are under way to close the remaining gaps; however, the sequence is of sufficient accuracy and contiguity to be declared substantially complete and to support an initial analysis of genome structure and preliminary gene annotation and interpretation. The genome encodes approximately 13,600 genes, somewhat fewer than the smaller Caenorhabditis elegans genome, but with comparable functional diversity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Transport / genetics
  • Chromatin / genetics
  • Cloning, Molecular
  • Computational Biology
  • Contig Mapping
  • Cytochrome P-450 Enzyme System / genetics
  • DNA Repair / genetics
  • DNA Replication / genetics
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism
  • Euchromatin
  • Gene Library
  • Genes, Insect
  • Genome*
  • Heterochromatin / genetics
  • Insect Proteins / chemistry
  • Insect Proteins / genetics
  • Insect Proteins / physiology
  • Nuclear Proteins / genetics
  • Protein Biosynthesis
  • Sequence Analysis, DNA*
  • Transcription, Genetic


  • Chromatin
  • Euchromatin
  • Heterochromatin
  • Insect Proteins
  • Nuclear Proteins
  • Cytochrome P-450 Enzyme System