Phospholipid and sterol analysis of plasma membranes of azole-resistant Candida albicans strains

FEMS Microbiol Lett. 2000 Apr 1;185(1):59-63. doi: 10.1111/j.1574-6968.2000.tb09040.x.


The phospholipid and sterol composition of the plasma membranes of five fluconazole-resistant clinical Candida albicans isolates was compared to that of three fluconazole-sensitive ones. The three azole-sensitive strains tested and four of the five resistant strains did not exhibit any major difference in their phospholipid and sterol composition. The remaining strain (R5) showed a decreased amount of ergosterol and a lower phosphatidylcholine:phosphatidylethanolamine ratio in the plasma membrane. These changes in the plasma membrane lipid and sterol composition may be responsible for an altered uptake of drugs and thus for a reduced intracellular accumulation of fluconazole thereby providing a mechanism for azole resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS-Related Opportunistic Infections / drug therapy
  • AIDS-Related Opportunistic Infections / microbiology
  • Antifungal Agents / pharmacology*
  • Antifungal Agents / therapeutic use
  • Candida albicans / chemistry
  • Candida albicans / drug effects*
  • Candidiasis / drug therapy
  • Candidiasis / microbiology
  • Cell Membrane / chemistry*
  • Drug Resistance, Microbial
  • Ergosterol / analysis*
  • Fluconazole / pharmacology*
  • Fluconazole / therapeutic use
  • Hematologic Neoplasms / drug therapy
  • Hematologic Neoplasms / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Phospholipids / analysis*


  • Antifungal Agents
  • Phospholipids
  • Fluconazole
  • Ergosterol