Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein

J Biochem. 2000 Jan;127(1):85-94. doi: 10.1093/oxfordjournals.jbchem.a022587.


Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a calculated molecular mass of 65.9 kDa. The predicted amino acid sequence shares 37.7, 41.8, and 39% identity with the sequences of yeast fimbrin, Arabidopsis thaliana fimbrin, and Dictyostelium discoideum plastin, respectively. The Tetrahymena F-actin-binding protein also shares two actin-binding domains previously identified in the fimbrin/plastin family, but lacks the EF-hand Ca2+-binding motif, suggesting that this protein is a novel-fimbrin-like protein in Tetrahymena. Moreover, we cloned a genomic DNA encoding the Tetrahymena fimbrin-like protein and performed Southern and Northern hybridizations. The results indicate that the genomic DNA possesses 9 introns and that both the gene and transcript of Tetrahymena fimbrin-like protein are single. Thus, we suggest that Tetrahymena fimbrin-like protein localizes to the division furrow and probably cross-links actin filaments in a Ca(2+)-insensitive manner during cytokinesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA, Bacterial / isolation & purification
  • DNA, Complementary / isolation & purification
  • Genes, Protozoan*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Microfilament Proteins / chemistry
  • Microfilament Proteins / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphoproteins / chemistry
  • Phylogeny
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tetrahymena thermophila / chemistry
  • Tetrahymena thermophila / genetics*
  • Transcription, Genetic


  • Carrier Proteins
  • DNA, Bacterial
  • DNA, Complementary
  • F-actin-binding proteins
  • Membrane Glycoproteins
  • Microfilament Proteins
  • Phosphoproteins
  • plastin

Associated data

  • GENBANK/AB024706